rat cryopreserved hepatocytes Search Results


rat (sprague-dawley) cryopreserved hepatocytes  (Thermo Fisher)
N/A
These plateable cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities. Cells per vial: 4-8 millionGet the Results You Need with GIBCO HepatocytesHigh quality hepatocytes,
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cryopreserved hepatocytes male sd rat  (BioIVT Inc)
90
BioIVT Inc cryopreserved hepatocytes male sd rat
Cryopreserved Hepatocytes Male Sd Rat, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat+hepatocytes/pmc09424906-144-0-42
Average 90 stars, based on 1 article reviews
cryopreserved hepatocytes male sd rat - by Bioz Stars, 2026-10
90/100 stars
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mouse, rat, dog, and monkey cryopreserved hepatocytes  (Corning Life Sciences)
90
Corning Life Sciences mouse, rat, dog, and monkey cryopreserved hepatocytes
Mouse, Rat, Dog, And Monkey Cryopreserved Hepatocytes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/mouse++rat++dog++and+monkey+cryopreserved+hepatocytes/pmc07558535-51-1-62
Average 90 stars, based on 1 article reviews
mouse, rat, dog, and monkey cryopreserved hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved rat, dog cynomolgus monkey primary hepatocytes  (PRIMACYT Cell Culture Technology GmbH)
90
PRIMACYT Cell Culture Technology GmbH cryopreserved rat, dog cynomolgus monkey primary hepatocytes
Cryopreserved Rat, Dog Cynomolgus Monkey Primary Hepatocytes, supplied by PRIMACYT Cell Culture Technology GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat++dog+cynomolgus+monkey+primary+hepatocytes/pmc10045298-56-4-11
Average 90 stars, based on 1 article reviews
cryopreserved rat, dog cynomolgus monkey primary hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved rat hepatocytes  (Verlag GmbH)
90
Verlag GmbH cryopreserved rat hepatocytes
Cryopreserved Rat Hepatocytes, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat+hepatocytes/pm24263968-396-23-0
Average 90 stars, based on 1 article reviews
cryopreserved rat hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved rat and mouse hepatocytes  (Yecuris Inc)
90
Yecuris Inc cryopreserved rat and mouse hepatocytes
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Rat And Mouse Hepatocytes, supplied by Yecuris Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat+and+mouse+hepatocytes/pmc09388686-175-3-11
Average 90 stars, based on 1 article reviews
cryopreserved rat and mouse hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved human and male wistar han rat hepatocytes a947  (BioIVT Inc)
90
BioIVT Inc cryopreserved human and male wistar han rat hepatocytes a947
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Human And Male Wistar Han Rat Hepatocytes A947, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+human+and+male+wistar+han+rat+hepatocytes+a947/pm38755248-594-12-17
Average 90 stars, based on 1 article reviews
cryopreserved human and male wistar han rat hepatocytes a947 - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved plateable primary wistar rat hepatocytes prh 3 males gzy r17g27 and 20k01  (BioIVT Inc)
90
BioIVT Inc cryopreserved plateable primary wistar rat hepatocytes prh 3 males gzy r17g27 and 20k01
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Plateable Primary Wistar Rat Hepatocytes Prh 3 Males Gzy R17g27 And 20k01, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+plateable+primary+wistar+rat+hepatocytes+prh+3+males+gzy+r17g27+and+20k01/pm38142784-54-0-39
Average 90 stars, based on 1 article reviews
cryopreserved plateable primary wistar rat hepatocytes prh 3 males gzy r17g27 and 20k01 - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved rat hepatocytes  (KU Leuven)
90
KU Leuven cryopreserved rat hepatocytes
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Rat Hepatocytes, supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat+hepatocytes/pm25828992-5-29-49
Average 90 stars, based on 1 article reviews
cryopreserved rat hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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pooled, female cryopreserved sprague–dawley rat, cynomolgus monkey and human hepatocytes  (BioIVT Inc)
90
BioIVT Inc pooled, female cryopreserved sprague–dawley rat, cynomolgus monkey and human hepatocytes
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Pooled, Female Cryopreserved Sprague–Dawley Rat, Cynomolgus Monkey And Human Hepatocytes, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/pooled++female+cryopreserved+sprague+dawley+rat++cynomolgus+monkey+and+human+hepatocytes/pm30386887-37-18-20
Average 90 stars, based on 1 article reviews
pooled, female cryopreserved sprague–dawley rat, cynomolgus monkey and human hepatocytes - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved rat hepatocytes m00005  (Celsis In Vitro Inc)
90
Celsis In Vitro Inc cryopreserved rat hepatocytes m00005
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Rat Hepatocytes M00005, supplied by Celsis In Vitro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+rat+hepatocytes+m00005/pm23089131-76-4-25
Average 90 stars, based on 1 article reviews
cryopreserved rat hepatocytes m00005 - by Bioz Stars, 2026-10
90/100 stars
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cryopreserved male sprague-dawley rat hepatocytes (n = 12)  (Becton Dickinson)
90
Becton Dickinson cryopreserved male sprague-dawley rat hepatocytes (n = 12)
Engraftment of wildtype rat <t>hepatocytes</t> rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.
Cryopreserved Male Sprague Dawley Rat Hepatocytes (N = 12), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+cryopreserved+hepatocytes/cryopreserved+male+sprague+dawley+rat+hepatocytes++n+++12+/pm30927663-43-2-11
Average 90 stars, based on 1 article reviews
cryopreserved male sprague-dawley rat hepatocytes (n = 12) - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Engraftment of wildtype rat hepatocytes rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.

Journal: Scientific Reports

Article Title: Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model

doi: 10.1038/s41598-022-18119-6

Figure Lengend Snippet: Engraftment of wildtype rat hepatocytes rescues lethal phenotype of FRG rats. ( a ) Kaplan–Meier survival curves of male and female Fah +/− (n = 33) and Fah −/− (n = 35F, 34M) rats after NTBC removal. ( b ) Serum ALT, AST, TBIL, ALP and ALB from terminal bleeds from 10 Fah +/− and 13 Fah −/− rats at day 14–20 after NTBC withdrawal. ( c ) H&E staining from one representative Fah +/− and Fah −/− showing extensive liver damage with NTBC withdrawal. ( d ) TaqMan real-time PCR data showing a decrease in hepatocyte-expressed genes (left) and an increase in fibrogenesis-related genes (right) in Fah −/− vs. Fah +/− rats. ( e ) Survival curve of Fah +/− (n = 9) rats or Fah −/− , with (n = 22) or without (n = 19) a transplant of wild-type rat hepatocytes, while following an NTBC cycling schedule. ( f ) Serum ALT, AST, TBIL, ALP and ALB levels of rats after sham surgery vs. rat hepatocyte transplant. ( g ) H&E and FAH IHC showing repopulation of the FRG rat liver with wild-type rat hepatocytes. ( h ) TaqMan data showing a rescue of gene changes in Fah −/− rats repopulated with wild-type hepatocytes vs. Fah −/− rats that received sham surgery, relative to Fah +/− rats with no liver damage (dotted line). Data in ( b ), ( d ) and ( f ) shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Unpaired t test.

Article Snippet: Cryopreserved rat and mouse hepatocytes were purchased from ThermoFisher (RTCP10) and Yecuris (20-0019), respectively.

Techniques: Staining, Real-time Polymerase Chain Reaction

Cross species engraftment of donor hepatocytes and liver humanization in FRG rats. ( a ) Engraftment of murine hepatocytes in FRG rats, shown by FAH IHC. ( b ) A highly engrafted humanized liver rat showing pale H&E staining characteristic of engrafted human hepatocytes, and confirmed by positive human albumin RNAscope staining and colocalization of FAH and human ASGR1 IHC staining. ( c ) Correlation of human albumin (mg/ml), human complement proteins, C3 and C5 (μg/ml), human TTR (μg/ml) and human AFP (ng/ml) in the serum of rats engrafted with primary human hepatocytes. ( d ) GS staining in FAH-positive hepatocytes around the central vein confirms that humanized livers maintain proper liver zonation.

Journal: Scientific Reports

Article Title: Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model

doi: 10.1038/s41598-022-18119-6

Figure Lengend Snippet: Cross species engraftment of donor hepatocytes and liver humanization in FRG rats. ( a ) Engraftment of murine hepatocytes in FRG rats, shown by FAH IHC. ( b ) A highly engrafted humanized liver rat showing pale H&E staining characteristic of engrafted human hepatocytes, and confirmed by positive human albumin RNAscope staining and colocalization of FAH and human ASGR1 IHC staining. ( c ) Correlation of human albumin (mg/ml), human complement proteins, C3 and C5 (μg/ml), human TTR (μg/ml) and human AFP (ng/ml) in the serum of rats engrafted with primary human hepatocytes. ( d ) GS staining in FAH-positive hepatocytes around the central vein confirms that humanized livers maintain proper liver zonation.

Article Snippet: Cryopreserved rat and mouse hepatocytes were purchased from ThermoFisher (RTCP10) and Yecuris (20-0019), respectively.

Techniques: Staining, RNAscope, Immunohistochemistry

Human-hepatocyte-specific gene delivery in humanized liver rats. ( a ) eGFP and FAH IHC show different staining patterns after AAV1 or AAV-NP59 mediated eGFP delivery to humanized livers. ( b ) AAV-NP59 shows preference for human hepatocytes, confirmed by double IHC for hASGR1 (blue) and eGFP (brown) in top panels, and eGFP or human albumin RNAscope (bottom panels). ( c ) Experiment timeline and pre-engraftment GFP expression in human hepatocytes (donor FCL) transduced with Lenti-GFP for 30 min at 5.00E + 04 viral genomes (VG)/cell in suspension. ( d ) hASGR1 and GFP single or double IHC in rat livers engrafted with lentivirus-infected, freshly-thawed human hepatocytes. ( e ) Pre-engraftment GFP expression in human hepatocytes (donor FCL) after 6 days in culture and a 1-hr infection with Lenti-GFP at 5.00E + 04 VG/cell. ( f ) FAH and GFP IHC staining confirms engraftment of lentivirus infected, cultured hepatocytes. Brown arrows point to GFP + human hepatocytes. Blue arrows mark GFP − human hepatocytes.

Journal: Scientific Reports

Article Title: Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model

doi: 10.1038/s41598-022-18119-6

Figure Lengend Snippet: Human-hepatocyte-specific gene delivery in humanized liver rats. ( a ) eGFP and FAH IHC show different staining patterns after AAV1 or AAV-NP59 mediated eGFP delivery to humanized livers. ( b ) AAV-NP59 shows preference for human hepatocytes, confirmed by double IHC for hASGR1 (blue) and eGFP (brown) in top panels, and eGFP or human albumin RNAscope (bottom panels). ( c ) Experiment timeline and pre-engraftment GFP expression in human hepatocytes (donor FCL) transduced with Lenti-GFP for 30 min at 5.00E + 04 viral genomes (VG)/cell in suspension. ( d ) hASGR1 and GFP single or double IHC in rat livers engrafted with lentivirus-infected, freshly-thawed human hepatocytes. ( e ) Pre-engraftment GFP expression in human hepatocytes (donor FCL) after 6 days in culture and a 1-hr infection with Lenti-GFP at 5.00E + 04 VG/cell. ( f ) FAH and GFP IHC staining confirms engraftment of lentivirus infected, cultured hepatocytes. Brown arrows point to GFP + human hepatocytes. Blue arrows mark GFP − human hepatocytes.

Article Snippet: Cryopreserved rat and mouse hepatocytes were purchased from ThermoFisher (RTCP10) and Yecuris (20-0019), respectively.

Techniques: Staining, RNAscope, Expressing, Transduction, Suspension, Infection, Immunohistochemistry, Cell Culture